Composite

Part:BBa_K4760000:Design

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


ClyA-PbrR-His6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The ClyA sequence is from E.coli K12 genome; the PbrR sequence is originated from Cupriavidus metallidurans CH34 strain. Both are synthesized and optimized to most preferred codon usage of E.coli BL21 strain.

References